Repository containing code for analysis of CRISPR-suppressor scanning experiments. Correspondence to Brian B. Liau (liau@chemistry.harvard.edu).
- CRISPR-suppressor_scanning_functions.py contains functions for analysis of sgRNA plasmid library and CRISPR-suppressor scanning sequencing data. See function docstrings for details regarding usage.
- Example_reference_file.csv is an example reference file containing sequences and annotations of library sgRNAs.
- Example_batch_file.csv is an example batch file for analysis of CRISPR-suppressor scanning data.
- Joung, J. et al. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat. Protoc. 12, 828–863 (2017).
- Canver, M. C. et al. Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments. Nat. Protoc. 13, 946–986 (2018).
- Vinyard, M. E. et al. CRISPR-suppressor scanning reveals a nonenzymatic role of LSD1 in AML. Nat. Chem. Biol. 15, 529–539 (2019).
- Gosavi, P. M. et al. Profiling the Landscape of Drug Resistance Mutations in Neosubstrates to Molecular Glue Degraders. ACS Cent. Sci. 8, 417–429 (2021).