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worm-psyqi

WormPsyQi is a semi-automated pipeline for high-throughput analysis of synapses in the nematode C. elegans. Using fluorescent protein-based techniques, WormPsyQi can quantitate multiple features of synapses, including number, size, intensity, and spatial distribution. We provide a pure python-based GUI.

Installation

Clone this repository and use the provided yml file to setup an Anaconda environment. If you're on a Windows machine, make sure that you run your Anaconda Prompt as administrator.

$ conda env create -f ./conda-environments/WORMPSYQI.yml

To check if the environment is installed successfully:

$ conda activate wormpsyqi

You will see your shell has (wormpsyqi) at the start as below.

(wormpsyqi) $ 

Run the pipeline

After installing and activating the environment, you can run WormPsyQi as below.

(wormpsyqi) $ python -m gui.main

See the running guide for more information.

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