This macro is used to generate a random distribution of small blue dots and bigger red circles. Some of these circles moreover are randomly filled in green.
Configuration dialog to generate sample images
Sample image with blue dots and red circles with and without green signal
Citation: Bosch, M., 2019. Introduction to ImageJ Macro Language in a Particle Counting Analysis: Automation Matters. Computer Optimized Microscopy: Methods and Protocols (Methods in Molecular Biology)
This macro analyses the staining of two proteins and a cellular organelle. The aim is to see how one of these two proteins colocalize with both the organelle and the other protein.
Membrane / Intracelular ratio of protein localization
Citation: Sastre, D. et al. 2019. Triple colocalization approach to assess traffic patterns and their modulation. Computer Optimized Microscopy: Methods and Protocols (Methods in Molecular Biology)
This macro analyses a pair of donor (D) and acceptor (A) images obtained from an intramolecular FRET biosensor. It generates an A/D ratio image and the histogram of this image.
Ratio image and histogram that result from the analysis of an active (above) and inactive (below) RacFRET biosensor using the FRETRatioAnalysis.ijm macro.
Image source: Kardash, E., et al. 2011. Imaging protein activity in live embryos using fluorescence resonance energy transfer biosensors. Nature Protocols.
Citation: Bosch, M. and Kardash, E. 2019. In vivo quantification of intramolecular FRET using RacFRET. Computer Optimized Microscopy: Methods and Protocols (Methods in Molecular Biology)