bulkToSingleRNAseq

Mapping zebrafish bulk RNA-seq data onto human scRNA-seq

This pipeline takes bulk RNAseq data from zebrafish liver cells obtained by FACS and compare to scRNA-seq data of human liver. First, one to one orthologs are identified between zebrafish and human. Then gene expression values are scaled in both bulk RNAseq samples and human scRNA-seq clusters. Top n most expressed or most variable genes between scRNA-seq clusters are chosen for comaprison. Scaled (z-score) CPM are correlated between zebrafish and human. It allows to match bulk data with scRNA-seq cluster corresponding to a given cell type.