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Given these two prefix adapters (plus some more so that the indexing logic is triggered) and allowing two errors:
^AAGCGCCAT
^AGGCATCAT
Cutadapt does not trim this query sequence at all:
ANGCATCATAAAAAAAAAA
And for this query, the first adapter is trimmed although the second one would have fewer errors:
AAGCATCATAAAAAAAAAA
Here are the alignments for the two adapters against the first query:
AAGCGCCAT
X XX
ANGCATCATAAAAAAAAAA
AGGCATCAT
X
ANGCATCATAAAAAAAAAA
There is a warning when creating the index that there are exist two sequences that cannot be matched uniquely. However, for the above sequences, it is clear which one is the better match.
This can lead to incorrect demultiplexing for adapters that can collide (even if the sequence that occurs in the read can unambiguously be assigned).
The text was updated successfully, but these errors were encountered:
Given these two prefix adapters (plus some more so that the indexing logic is triggered) and allowing two errors:
Cutadapt does not trim this query sequence at all:
And for this query, the first adapter is trimmed although the second one would have fewer errors:
Here are the alignments for the two adapters against the first query:
There is a warning when creating the index that there are exist two sequences that cannot be matched uniquely. However, for the above sequences, it is clear which one is the better match.
This can lead to incorrect demultiplexing for adapters that can collide (even if the sequence that occurs in the read can unambiguously be assigned).
The text was updated successfully, but these errors were encountered: