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sonegop committed Sep 14, 2018
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19 changes: 15 additions & 4 deletions command/docs/use_cases.rst
Original file line number Diff line number Diff line change
Expand Up @@ -158,6 +158,10 @@ Import Gene Annotation
Since the platforms related to the selected experiment were never imported before into COMMAND>_, we need the gene sequences in order to properly import our probes at gene level. We got gene/sequence list from ncbi: go `here <https://www.ncbi.nlm.nih.gov/nuccore/AP007209.1>`_ and from the top-right button select send to: Coding sequences, Format: FASTA Nucleotide and Choose destination: File.
In COMMAND>_ go to > Data Collection (on the top left corner) then > Bio features (genes) > Import biological feature (+ symbol on the bottom left) > Type: FASTA , File name: select the annotation file you downloaded before > Import Biological features.

.. _Case03_01:
.. figure:: _static/Case03_01.png
:align: center

Parse Platforms and Samples
+++++++++++++++++++++++++++

Expand All @@ -176,14 +180,21 @@ In Experiment files Section > File Assignement > Select the .txt files (all Sult
- Parameters: ch1
- Only selected files checked

Platform tab > Script: > `gpr_sample.py`; parameters: Gene name,Spot Mean Intensity (Cyanine5_060909_1136(1)),0; Execution; order: 2
Platform tab

- Script: `gpr_sample.py`
- parameters: Gene name,Spot Mean Intensity (Cyanine5_060909_1136(1)),0
- Execution order: 2

Do the same again for the ch2 but use as Parameters for Platform:

DO the same again for the ch2 but use as Parameters for Platform:
Platform tab

Platform tab > Script: > `gpr_sample.py`; parameters: Gene name,Spot Mean Intensity (Cyanine3_060909_1136(1)),0; Execution; order: 2
- Script: `gpr_sample.py`
- parameters: Gene name,Spot Mean Intensity (Cyanine3_060909_1136(1)),0
- Execution order: 2

for GPL10439:
for Platform GPL10439:

- In Experiment files Section > File Assignement > Select the .ndf file and on the Assign files dialog":

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