The smDynamicsAnalyzer is an open-source macro that can determine a series of dynamics parameters from the single-molecule tracking dataset simply by following the designated steps after building an appropriate folder structure.
Refer to the Ref 1 for the detailed instructions.
Check the supplementary figures below as well.
One may freely customize and use the macro with suitable citations of Ref 1.
Igor Pro 8
https://www.wavemetrics.com/products/igorpro
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Yanagawa M., Sako Y. (2021) Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR. In: Kim SB. (eds) Live Cell Imaging. Methods in Molecular Biology, vol 2274. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1258-3_32
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Yanagawa M, Hiroshima M, Togashi Y, Abe M, Yamashita T, Shichida Y, Murata M, Ueda M, and Sako Y. (2018) Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors. Sci Signal 11(548). doi:10.1126/scisignal.aao1917 https://stke.sciencemag.org/content/11/548/eaao1917
The toll free refer links for Ref. 2 are available on the HP of Cellular Informatics Lab., Riken CPR. http://www2.riken.jp/cell-info/publication/
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Copyright (c) 2020- Masataka Yanagawa.
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