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make trimming adaptor sequence configurable, maybe skip TrimGalore #151

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steffenheyne opened this issue Jun 12, 2018 · 5 comments
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@steffenheyne
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see dicussion here:
#53

@dpryan79 dpryan79 changed the title make trimming adaptor sequence configurable, maybe skeip TrimGalore make trimming adaptor sequence configurable, maybe skip TrimGalore Jun 12, 2018
@dpryan79
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dpryan79 commented Jul 4, 2018

An alternative would be fastp. Apparently it's multithreaded and can do a bunch of nice things like filtering by complexity and poly-A content. Some of the Galaxy tutorials might get switched over to this.

@dpryan79
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dpryan79 commented Sep 10, 2018

In case anyone external to us is wondering, I've benchmarked fastp and it's slower than cutadapt once you start using more than ~5 cores. I really like some of its options, so I'm currently trying to figure out if I can speed it up a bit.

@mictadlo
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mictadlo commented Oct 3, 2018

What about afterqc?

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dpryan79 commented Oct 3, 2018

@mictadlo My understanding is that afterqc is quite similar, only slower. It's from the same authors and they mention that in the fastp preprint at least. I have to admit to not having tried afterqc, though. If you've found it to be performant and the results to be good then let me know :)

@mictadlo
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mictadlo commented Oct 4, 2018

Thank you for pointing it out to me. fastp sounds good actually.

@dpryan79 dpryan79 closed this as completed Jun 4, 2019
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