Example for barcodes files.

[fanyue322]

Hi

Thanks for your great tool.

I want to perform ASE calling from 10X scRNA-seq data. Is there an example file for barcodes file(barcodes and their group/cell mapping) so that I can follow the tutorial? Many thanks.

Best

[AaronJeeHo]

Hello,

Thank you the interest in the software!

The barcode file is a two column tsv with the first column denoting barcode, and the second barcode denoting some form of grouping (ie. Cell-Type / Clusters). This file should not include a header.

Format...

CACCCAAGTGAGTTGG-1	Oligodendrocytes
GCTTAAGCCGCGGCAT-1	Oligodendrocytes
GTCACGGGTGGCCTAG-1	Endothelial
GCGATCGAGGCTTAAA-1	Oligodendrocytes
TACTCGCGTACGGTTG-1	OPC

Since the software is still very much in pre-development, the single cell pipeline in particular will go through a lot of changes. In the future it hopefully should be easier to work with 10x data, and the required inputs may change.

A formal tutorial with examples, seem like a useful thing to have. I'll look to include these in the future as I continue to improve the documentation.

Thanks!