mesoSPIM Version 5
What is the mesoSPIM project?
The mesoSPIM (mesoscale selective plane illumination microscopy) project aims at creating open-hardware microscopy platforms for imaging in cleared tissue. Core features of the current generation mesoSPIM setups are:
- compatibilty with all major clearing techniques including CLARITY and iDISCO
- large field-of-views, ranging from 2 to 20 mm using an Olympus MVX-10 macroscope
- dual-sided excitation
- optimized for fast screening of whole mouse brain samples, yielding relatively small datasets (10-14 GB/brain/color)
- near-isotropic sampling (5-10 µm in X,Y & Z) due to an axially swept lightsheet which leads to uniform z-resolution across the field-of-view
- modular sample holders and quick sample exchange
- large travel range (45 x 45 x 100 mm) to allow imaging of very large samples
The mesoSPIM wiki
The mesoSPIM wiki provides documentation, setup, and usage instructions.
Who is the mesoSPIM project for?
- You are looking for a versatile imaging platform for cleared tissue that can be tailored to your needs.
- Research groups and imaging facilities with experience in building and supporting custom microscopes.
What are ideal imaging applications for a mesoSPIM?
- screening of large numbers of samples for clearing & labeling quality
- visualization of cell populations or other structures (blood vessels, plaques) in whole cleared organs
What are the mesoSPIM instruments less well suited for?
- imaging live samples (this is the domain of classical light-sheet microscopes)
- high-resolution (<1 µm pixelsize) scans with large number of tiles across whole mouse brains (this is better done using light-sheet instruments not based on a zoom macroscopes such as the Zeiss Z.1., the COLM)