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Update assembly.rst
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nkleinbo committed Nov 13, 2017
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6 changes: 3 additions & 3 deletions docs/assembly_qc/assembly.rst
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Expand Up @@ -76,15 +76,15 @@ We will run the assembly in two steps.
Generate corrected reads
------------------------

Command::
The correction stage selects the best overlaps to use for correction, estimates corrected read lengths, and generates corrected reads::

canu -correct -d ~/canu_correct -p 'canuAssembly' 'genomeSize=3m' 'gnuPlotTested=true' 'useGrid=false' 'maxThreads=16' 'minThreads=16' -nanopore-raw D1_basecall/workspace/pass/
canu -correct -d ~/canu_correct -p 'canuAssembly' 'genomeSize=3m' 'gnuPlotTested=true' 'useGrid=false' -nanopore-raw 1D_basecall.fastq


Genereate and assemble trimmed reads
-----------------------

Command::
The trimming stage identifies unsupported regions in the input and trims or splits reads to their longest supported range. The assembly stage makes a final pass to identify sequencing errors; constructs the best overlap graph (BOG); and outputs contigs, an assembly graph, and summary statistics::

canu -trim-assemble -d ~/canu_assembly -p 'canuAssembly' 'genomeSize=3M' 'useGrid=false' 'minThreads=16' 'maxThreads=16' 'gnuPlotTested=true' -nanopore-corrected ~/canu_correct/canuAssembly.correctedReads.fasta.gz -nanopore-corrected ~/D1_2_basecall/workspace/fastq_runid_*
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