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reads2transcriptome

From second generation sequencing reads to nucleotide counts This small pipeline works with single illumina reads (no PE).

Usage

  • make all

Notes

  • You may want to run make qc first and use its results to tweak the trimming parameters
  • By default the TrueSeq3 adapters will be trimmed by Trimmomamtic

Prerequisites

  • Python + Biopython
  • FastQC
  • Kraken
  • Trimmomatic
  • kallisto

Copyright

Copyright (C) <2015> EMBL-European Bioinformatics Institute

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.

Neither the institution name nor the name reads2transcriptome can be used to endorse or promote products derived from this software without prior written permission. For written permission, please contact marco@ebi.ac.uk.

Products derived from this software may not be called reads2transcriptome nor may reads2transcriptome appear in their names without prior written permission of the developers. You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.

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