Error in assay(object, i = exprs_values, ...) : 'assay(<SingleCellExperiment>, i="character", ...)' invalid subscript 'i'

[yueli8]

Hello miaozhun,

Thank you so much for developing so nice software!

May I ask a quick question?

Thank you in advance for your great help!

Best,

Yue

> Dendritic.sce<-as.SingleCellExperiment(Dendritic)
> group<-factor(c(rep(1,2165),rep(2,363)))
> results<-DEsingle(counts=NKT.sec,group=group)
Error in assay(object, i = exprs_values, ...) : 
  'assay(<SingleCellExperiment>, i="character", ...)' invalid subscript 'i'
'counts' not in names(assays(<SingleCellExperiment>))
[Dendritic.zip](https://github.com/miaozhun/DEsingle/files/11120698/Dendritic.zip)

[yueli8]

library(Seurat)
library(scHCL)
###这里直接使用Seurat分析结果后的对象保存的rds文件
rds<-readRDS('cluster.ifnb.rds')
counts<-as.matrix(rds@assays$RNA@counts) ##官网建议的是Rawcounts
dim(counts)
#[1] 2884 80
https://www.jianshu.com/p/c88c045b0c28