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This is the file with codes for analysis done in the research article Cultivation and antimicrobial screening of sponge-associated bacteria from the marine sponges Agelas sventres and Xestospongia muta collected from different depths submitted to Marine Drugs Journal.

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Indraningrat-et-al.-CultivationSpongedepths2019

Study title: "Cultivation and antimicrobial screening of sponge-associated bacteria from the marine sponges Agelas sventres and Xestospongia muta collected from different depths" Authors: Anak Agung Gede Indraningrat, Sebastian Micheller, Mandy Runderkamp, Ina Sauerland1, Leontine. E. Becking, Hauke Smidt and Detmer Sipkema

In this repository are all the files and codes used for reproducible data analysis Below is a short descriptions of the files.

#Complete workflow

Manuscript_cultivation.rmd contains -R Markdown file containing all details and analysis scripts used in this study.

#Biom File

Cultivation_final.biom provides 16S rRNA gene amplicon sequencing data, classified to Genus level (SILVA SSU 128 database)

#Mapping File

Mapping_cultivation.csv contains metadata for individual samples

#Phyloggenetic tree

DG.tre contains phylogenetic tree information of OTUs.

#Heatmap

HeatMap.csv contains list of OTUs with average relative abundance above 0.25% in all samples.

All questions or comments concerning this analysis can be directed at indraningrat@warmadewa.ac.id

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This is the file with codes for analysis done in the research article Cultivation and antimicrobial screening of sponge-associated bacteria from the marine sponges Agelas sventres and Xestospongia muta collected from different depths submitted to Marine Drugs Journal.

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