Minor Update

This release contains minor changes:

• adds a general aligned/unaligned pie chart
• adds Docker support:

To build your container clone the repository and run docker build -t sequ-into . .
After this has successfully finished, you can run sequ-into via Docker docker run -v D:/data:/mnt/d -it -e DISPLAY=$XSERVER sequ-into . You need to mount the folders you want to access with the -voption, and should also provide a $XSERVER location, e.g. 192.168.2.101:0.0 such that the GUI can be shown.

Linux and Mac OS users may use the docker build, a Windows executable of this release is attached.

Demo data extension

The demo data have been extended with demo use-cases 6 and 7. In use-case 6 simulated reads are used to assess the accuracy of the mapping capabilities of the methods employed by sequ-into, while use-case 7 (ERR3078229-ERR3078231) is a meta-genomics benchmark to assess how well sequ-into assigns reads to a specific organism.

sequ-into v1.3.0 (multi-FAST5, incremental stats)

Updating sequ-into to the newest release.

This release now features:

• online-algorithm architecture for incremental analysis
• multi-FAST5 files
• supports analysis while sequencing (add ONT tmp folder; requires FASTQ output enabled)
• easy detection of ribosomal RNA using the included riboseq-Library
• easily share-able HTML report of your analysis

We also provide a set of videos show-casing the use of sequ-into: https://www.youtube.com/playlist?list=PLwTdrzBYdV7nvT-eTiPZ37StFHqsL8e82

On Windows simply download the sequ-into-1.3.0.exe and double-click to run.
On Mac OS download the sequ-into-1.3.0.dmg and pull the App into your Applications folder. Do not start sequ-into from the dmg, since DMGs are write-protected ...
On Ubuntu/Debian use the provided AppImage or deb package.

PLEASE FOLLOW THE INSTALLATION INSTRUCTIONS https://sequ-into.readthedocs.io/en/latest/install.html

On Windows you will need Windows Subsystem for Linux (free onboard the professional version)!

If you are using Linux (non Ubuntu/non-Debian) please clone this repository and build sequ-into yourself.

Test/Demo data is provided within this release (sequ_into_demo.zip). They are also available from figshare .

sequ-into supporting multi-FAST5 and tmp-Files

Updating sequ-into to the newest release.

This release now features:

• multi-FAST5 files
• supports analysis while sequencing (add ONT tmp folder)
• easy detection of ribosomal RNA using the included riboseq-Library
• easily share-able HTML report of your analysis

We also provide a set of videos show-casing the use of sequ-into: https://www.youtube.com/playlist?list=PLwTdrzBYdV7nvT-eTiPZ37StFHqsL8e82

On Windows simply download the sequ-into-1.2.0.exe and double-click to run.
On Mac OS download the sequ-into-1.2.0.dmg and pull the App into your Applications folder. Do not start sequ-into from the dmg, since DMGs are write-protected ...

PLEASE FOLLOW THE INSTALLATION INSTRUCTIONS https://sequ-into.readthedocs.io/en/latest/install.html

On Windows you will need Windows Subsystem for Linux (free onboard the professional version)!

If you are using Linux, please raise an issue and we will provide you with an executable ;) You may also build it yourself :)

We will (hopefully) be able to provide the multi-FAST5 demo-data here soon. Until then please use the old demo-data from the previous release :)

sequ-into v1.1.0 Release

Mac OS Users please download sequ-into-1.1.0.dmg.
Windows Users please download sequ-into.1.1.0.exe.

Linux users please follow the manual build instructions in our docs. If there is demand for pre-built binaries of sequ-into for Linux we will consider providing them, too.

If you want to test sequ-into, we also provide demo-data for download ( demo_data_sequinto.zip ).