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AVANT WP1 FMT trial

This is the repository for R scripts used in the analysis of 16S rRNA microbiome data analysis generated in the FMT trial from the EU Innovation Action project AVANT.

updated 11/06/2023, Mattia Pirolo

Objective

To examine the impact of transplanting feces or gastric content on health and growth parameters and paraclinical parameters in recipient single-housed piglets exposed to a weaning transition and challenged with enterotoxigenic Escherichia coli (ETEC).

Study design

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Data

This folder contains all datafiles:

  • ps_FMT.rds: phyloseq object in R data format for the analysis
  • metadata.tsv: tab-separated values file with relevant metadata for the analysis

R scripts

This folder contains all R scripts:

  • composition.R: R script for visualization of taxonomic composition
  • diversity.R: R script for alpha- and beta-diversity analysis
  • DESeq2.R: R script for differential abundance analysis using DESeq2

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