guppy_basecaller_duplex generated six fastq files from 252 fast5 files....

[MostafaYA]

Hi,
I used guppy_basecaller_duplex on data produced with the kit SQK-NBD112-24 on FLO-MIN112 flowcell.

The Following error appears and out of 252 fast5 files, ONLY six fastq files were produced. That I feel is a big loss of data if this
is ok.
This was the error "repeatd many many times with different sample id"
[guppy/error] basecalling::PriorityReadQueue::pass_read_pair: Cannot process duplex read pair 9b230638-706f-43b7-a1b4-f2ff63fe410d, 4a1468c9-0093-40b5-8785-328b331cff11 as sample count 324630 is over maximum supported by caller of 160000, putting on output queue

Despite the above error, guppy_basecaller_duplex said Basecalling was successful
********* Caller time: 1135416 ms, Samples called: 185914147, samples/s: 163741 Finishing up any open output files. Basecalling completed successfully.

This was my call
guppy_basecaller_duplex -i fast5_pass/ -r -s duplex_calls -x auto --kit SQK-NBD112-24 --flowcell FLO-MIN112 --chunks_per_runner 16 --duplex_pairing_mode from_pair_list --duplex_pairing_file duplex_output/pair_ids_filtered.txt --trim_barcodes --compress_fastq

NB. The config file q20-fixed-2.0-ft-10M.cfg was not available is guppy version 6.1.2+e0556ff. Hence I used flags --kit and --flowcell. Also I used the config file dna_r10.4_e8.1_sup.cfg. No imporovement

[cjw85]

Hi @MostafaYA,

You are better off emailing support@nanoporetech.com or asking this question in the Nanopore Community; it is a different team internally that supports Guppy than those which develop and can support this duplex_tools project.