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Error with alignment to reference in gene_expression tutorial #30

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john-hancock opened this issue Aug 5, 2021 · 0 comments
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@john-hancock
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I'm running the gene_expression tutorial on a MacBook Air with the Apple chip.

I get the following error:

Error in rule build_minimap_index:
jobid: 0
output: index/transcriptome_index.mmi
shell:

    minimap2 -t 4  -I 1000G -d index/transcriptome_index.mmi /epi2melabs/differential-expression/sample_data/Homo_sapiens.GRCh38.cdna.all.fa.gz

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)

Is there a solution to this? I have just run the tutorial straightforwardly without any tinkering so it looks like a scripting/parameterization issue

Full log:

Building DAG of jobs...
Using shell: /usr/bin/bash
Provided cores: 4
Rules claiming more threads will be scaled down.
Conda environments: ignored
Job counts:
count jobs
1 build_minimap_index
1

[Thu Aug 5 10:42:13 2021]
rule build_minimap_index:
input: /epi2melabs/differential-expression/sample_data/Homo_sapiens.GRCh38.cdna.all.fa.gz
output: index/transcriptome_index.mmi
jobid: 0
threads: 4

[Thu Aug 5 10:42:32 2021]
Error in rule build_minimap_index:
jobid: 0
output: index/transcriptome_index.mmi
shell:

    minimap2 -t 4  -I 1000G -d index/transcriptome_index.mmi /epi2melabs/differential-expression/sample_data/Homo_sapiens.GRCh38.cdna.all.fa.gz

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)

Removing output files of failed job build_minimap_index since they might be corrupted:
index/transcriptome_index.mmi
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message
Complete log: /epi2melabs/differential-expression/.snakemake/log/2021-08-05T104211.761394.snakemake.log

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