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Snakefile
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Snakefile
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rule all:
input:
auspice_tip_frequencies = "auspice/zika_tip-frequencies.json",
auspice_v1_tree = "auspice/v1_zika_tree.json",
auspice_v1_meta = "auspice/v1_zika_meta.json",
auspice_v1_seq = "auspice/v1_zika_seq.json",
auspice_v1_tip_frequencies = "auspice/v1_zika_tip-frequencies.json",
auspice_v2 = "auspice/v2_zika.json",
auspice_v2_tip_frequencies = "auspice/v2_zika_tip-frequencies.json"
rule files:
params:
input_fasta = "data/zika.fasta",
dropped_strains = "config/dropped_strains.txt",
reference = "config/zika_outgroup.gb",
colors = "config/colors.tsv",
auspice_config_v1 = "config/auspice_config_v1.json",
auspice_config_v2 = "config/auspice_config_v2.json"
files = rules.files.params
rule parse:
input:
sequences = files.input_fasta
output:
sequences = "results/sequences.fasta",
metadata = "results/metadata.tsv"
params:
fasta_fields = "strain virus accession date region country division city db segment authors url title journal paper_url",
prettify_fields = "region country division city"
shell:
"""
augur parse \
--sequences {input.sequences} \
--output-sequences {output.sequences} \
--output-metadata {output.metadata} \
--fields {params.fasta_fields} \
--prettify-fields {params.prettify_fields}
"""
rule filter:
message: "Filtering sequences"
input:
sequences = rules.parse.output.sequences,
metadata = rules.parse.output.metadata,
exclude = files.dropped_strains
output:
sequences = "results/filtered.fasta"
params:
group_by = "country year month",
sequences_per_group = 1,
min_date = 2012
shell:
"""
augur filter \
--sequences {input.sequences} \
--metadata {input.metadata} \
--exclude {input.exclude} \
--output {output.sequences} \
--group-by {params.group_by} \
--sequences-per-group {params.sequences_per_group} \
--min-date {params.min_date}
"""
rule align:
message: "Aligning sequences"
input:
sequences = rules.filter.output.sequences,
reference = files.reference
output:
alignment = "results/aligned.fasta"
shell:
"""
augur align \
--sequences {input.sequences} \
--reference-sequence {input.reference} \
--output {output.alignment} \
--fill-gaps
"""
rule tree:
message: "Building tree"
input:
alignment = rules.align.output.alignment
output:
tree = "results/tree_raw.nwk"
params:
method = "iqtree"
shell:
"""
augur tree \
--alignment {input.alignment} \
--output {output.tree} \
--method {params.method}
"""
rule refine:
message: "Refining tree"
input:
tree = rules.tree.output.tree,
alignment = rules.align.output,
metadata = rules.parse.output.metadata
output:
tree = "results/tree.nwk",
node_data = "results/branch_lengths.json"
params:
coalescent = "opt",
date_inference = "marginal",
clock_filter_iqd = 4
shell:
"""
augur refine \
--tree {input.tree} \
--alignment {input.alignment} \
--metadata {input.metadata} \
--output-tree {output.tree} \
--output-node-data {output.node_data} \
--timetree \
--coalescent {params.coalescent} \
--date-confidence \
--date-inference {params.date_inference} \
--clock-filter-iqd {params.clock_filter_iqd}
"""
rule ancestral:
message: "Reconstructing ancestral sequences and mutations"
input:
tree = rules.refine.output.tree,
alignment = rules.align.output
output:
node_data = "results/nt_muts.json"
params:
inference = "joint"
shell:
"""
augur ancestral \
--tree {input.tree} \
--alignment {input.alignment} \
--output {output.node_data} \
--inference {params.inference}
"""
rule translate:
message: "Translating amino acid sequences"
input:
tree = rules.refine.output.tree,
node_data = rules.ancestral.output.node_data,
reference = files.reference
output:
node_data = "results/aa_muts.json"
shell:
"""
augur translate \
--tree {input.tree} \
--ancestral-sequences {input.node_data} \
--reference-sequence {input.reference} \
--output {output.node_data} \
"""
rule traits:
message: "Inferring ancestral traits"
input:
tree = rules.refine.output.tree,
metadata = rules.parse.output.metadata
output:
node_data = "results/traits.json",
params:
columns = "region country",
sampling_bias_correction = 3
shell:
"""
augur traits \
--tree {input.tree} \
--metadata {input.metadata} \
--output {output.node_data} \
--columns {params.columns} \
--sampling-bias-correction {params.sampling_bias_correction} \
--confidence
"""
rule export_v1:
message: "Exporting data files for for auspice using nextflu compatible schema"
input:
tree = rules.refine.output.tree,
metadata = rules.parse.output.metadata,
branch_lengths = rules.refine.output.node_data,
traits = rules.traits.output.node_data,
nt_muts = rules.ancestral.output.node_data,
aa_muts = rules.translate.output.node_data,
colors = files.colors,
auspice_config = files.auspice_config_v1
output:
auspice_tree = rules.all.input.auspice_v1_tree,
auspice_meta = rules.all.input.auspice_v1_meta,
auspice_seq = rules.all.input.auspice_v1_seq
shell:
"""
augur export v1 \
--tree {input.tree} \
--metadata {input.metadata} \
--node-data {input.branch_lengths} {input.traits} {input.nt_muts} {input.aa_muts} \
--colors {input.colors} \
--auspice-config {input.auspice_config} \
--output-tree {output.auspice_tree} \
--output-meta {output.auspice_meta} \
--output-sequence {output.auspice_seq}
augur validate export-v1 {output.auspice_meta} {output.auspice_tree}
"""
rule export_v2:
message: "Exporting data files for for auspice using nextstrain schema v2"
input:
tree = rules.refine.output.tree,
metadata = rules.parse.output.metadata,
branch_lengths = rules.refine.output.node_data,
traits = rules.traits.output.node_data,
nt_muts = rules.ancestral.output.node_data,
aa_muts = rules.translate.output.node_data,
auspice_config = files.auspice_config_v2,
colors = files.colors
params:
title = "Real-time tracking of Zika virus evolution -- v2 JSON"
output:
auspice_main = rules.all.input.auspice_v2
shell:
"""
augur export v2 \
--tree {input.tree} \
--metadata {input.metadata} \
--node-data {input.branch_lengths} {input.traits} {input.nt_muts} {input.aa_muts} \
--colors {input.colors} \
--auspice-config {input.auspice_config} \
--output {output.auspice_main} \
--title {params.title:q} \
--panels tree map entropy frequencies
"""
rule tip_frequencies:
input:
tree = rules.refine.output.tree,
metadata = rules.parse.output.metadata
params:
pivot_interval = 3
output:
tip_freq = rules.all.input.auspice_tip_frequencies
shell:
"""
augur frequencies \
--method kde \
--tree {input.tree} \
--metadata {input.metadata} \
--pivot-interval {params.pivot_interval} \
--output {output}
"""
rule copy_tip_frequencies:
input:
frequencies = rules.tip_frequencies.output.tip_freq
output:
v1 = rules.all.input.auspice_v1_tip_frequencies,
v2 = rules.all.input.auspice_v2_tip_frequencies
shell:
"""
cp -f {input} {output.v1};
cp -f {input} {output.v2};
"""
rule clean:
message: "Removing directories: {params}"
params:
"results ",
"auspice"
shell:
"rm -rfv {params}"