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There is no qiime2 result file in the results #745

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petemeng opened this issue May 29, 2024 · 2 comments
Closed

There is no qiime2 result file in the results #745

petemeng opened this issue May 29, 2024 · 2 comments

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@petemeng
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Description of the bug

There is no qiime2 result file in my results.

1716991446485

Command used and terminal output

nextflow run ampliseq/main.nf \
-profile conda \
--input manifest_new \
--FW_primer ACTCCTACGGGAGGCAGCA \
--RV_primer GGACTACHVGGGTWTCTAAT \
--metadata metadata_new.tsv \
--outdir ./results2 \
--metadata_category condition \
--metadata_category_barplot condition \
--picrust true \
--skip_qiime false


### Relevant files

The resulting file is as follows:
barrnap
cutadapt
dada2
fastqc
input
multiqc
overall_summary.tsv
phyloseq
pipeline_info
summary_report

### System information

Nextflow 23.4.2
ampliseq 2.9.0
@petemeng petemeng added the bug Something isn't working label May 29, 2024
@d4straub
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Hi there,
the pipeline cannot execute QIIME2 with conda.
You should have gotten at least one big warning saying
"QIIME2 does not support Conda. Please use Docker / Singularity / Podman instead."
did you not?
It is also discouraged to use conda, see https://nf-co.re/ampliseq/2.9.0/docs/usage#-profile
Other than that, maybe it would be good to add that warning somewhere in the documentation. Where would you have seen it?

@d4straub d4straub removed the bug Something isn't working label May 29, 2024
@petemeng
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oh! Thank you very much!!!

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