main.nf

// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'

params.options = [:]
options        = initOptions(params.options)

process BWA_MEM {
    tag "$meta.id"
    label 'process_high'
    publishDir "${params.outdir}",
        mode: params.publish_dir_mode,
        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }

    conda (params.enable_conda ? "bioconda::bwa=0.7.17 bioconda::samtools=1.12" : null)
    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
        container "https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:66ed1b38d280722529bb8a0167b0cf02f8a0b488-0"
    } else {
        container "quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:66ed1b38d280722529bb8a0167b0cf02f8a0b488-0"
    }

    input:
    tuple val(meta), path(reads)
    path  index

    output:
    tuple val(meta), path("*.bam"), emit: bam
    path  "*.version.txt"         , emit: version

    script:
    def split_cpus = Math.floor(task.cpus/2)
    def software   = getSoftwareName(task.process)
    def prefix     = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
    def read_group = meta.read_group ? "-R ${meta.read_group}" : ""
    """
    INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`

    bwa mem \\
        $options.args \\
        $read_group \\
        -t ${split_cpus} \\
        \$INDEX \\
        $reads \\
        | samtools view $options.args2 -@ ${split_cpus} -bhS -o ${prefix}.bam -

    echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//' > ${software}.version.txt
    """
}