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I am trying to run rnaseq but it keeps failing at the same step: NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
It fails giving an exit status of 1, but no error message seems to be detailing anything wrong in the main log file besides the warning: WARNING: Skipping mount /var/apptainer/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container
I'm running this on our cluster using slurm. I've also been using the singularity profile.
Command used and terminal output
Main command to launch rnaseq:sbatch << EOF#!/bin/bash -e#SBATCH --job-name=SAM_RNA#SBATCH --cpus-per-task=16#SBATCH --mem=32G#SBATCH --time=23:59:59module load nextflow/22.10.4#nextflow pull nf-core/rnaseqnextflow run nf-core/rnaseq --input $SAMPLESHEET --outdir $OUTDIR --fasta $INPUT_GENOME --gff $GENOME_ANNOTATION --clip_r1=1 --clip_r2=1 -profile singularity#nextflow run nf-core/rnaseq -profile test,singularity --outdir $OUTDIREOF_________________________________________________________________________________________Error message in the log file (which is repeated multiple times in the log file at different points):-[nf-core/rnaseq] Pipeline completed with errors-Error executing process > 'NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (142_SEX-FEMALE_STAGE-5)'Caused by: Process `NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION (142_SEX-FEMALE_STAGE-5)` terminated with an error exit status (1)Command executed: junction_annotation.py \ -i 142_SEX-FEMALE_STAGE-5.markdup.sorted.bam \ -r fixed_new_annotation.bed \ -o 142_SEX-FEMALE_STAGE-5 \ \ 2> 142_SEX-FEMALE_STAGE-5.junction_annotation.log cat <<-END_VERSIONS > versions.yml "NFCORE_RNASEQ:RNASEQ:BAM_RSEQC:RSEQC_JUNCTIONANNOTATION": rseqc: $(junction_annotation.py --version | sed -e "s/junction_annotation.py //g") END_VERSIONSCommand exit status: 1Command output: total = 13227417Command error: INFO: Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred INFO: Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred WARNING: Skipping mount /var/apptainer/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in containerWork dir: /powerplant/workspace/cfnsjm/my_files/C_auratus/transcriptomics/rnaseq/work/85/84ceed6f2e2bb7af85ac1608b77ad9Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
===================================================================
Total splicing Events: 11244137
Known Splicing Events: 10461617
Partial Novel Splicing Events: 226475
Novel Splicing Events: 543000
Filtered Splicing Events: 13045
Traceback (most recent call last):
File "/usr/local/bin/junction_annotation.py", line 171, in
main()
File "/usr/local/bin/junction_annotation.py", line 149, in main
obj.annotate_junction(outfile=options.output_prefix,refgene=options.ref_gene_model,min_intron=options.min_intron, q_cut = options.map_qual)
File "/usr/local/lib/python3.7/site-packages/qcmodule/SAM.py", line 3832, in annotate_junction
(chrom, i_st, i_end) = i.split(":")
ValueError: too many values to unpack (expected 3)
SamMod1
changed the title
RSEQC_JUNCTIONANNOTATION terminated with an error exit status (1)
RSEQC_JUNCTIONANNOTATION ERROR: too many values to unpack
Jul 26, 2023
SamMod1
changed the title
RSEQC_JUNCTIONANNOTATION ERROR: too many values to unpack
RSEQC_JUNCTIONANNOTATION error: too many values to unpack
Jul 26, 2023
Issue fixed. junction_annotation.py errors if there are colons ':' in the sequence id field of a gff. I fixed the issue by replacing all of the ':' in the sequence id column with underscores.
Description of the bug
I am trying to run rnaseq but it keeps failing at the same step: NFCORE_RNASEQ:RNASEQ:RSEQC:RSEQC_JUNCTIONANNOTATION
It fails giving an exit status of 1, but no error message seems to be detailing anything wrong in the main log file besides the warning: WARNING: Skipping mount /var/apptainer/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container
I'm running this on our cluster using slurm. I've also been using the singularity profile.
Command used and terminal output
Relevant files
slurm-1729803.txt
nextflow.log
System information
Nextflow:
nextflow/22.10.4
rmaseq:
nf-core/rnaseq v3.12.0-g3bec233
Hardware:
Slurm Cluster
Profile:
Singularity
OS:
"CentOS Linux"
VERSION="7 (Core)"
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