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Update salmon_tutorial.rst
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halexand committed Oct 12, 2016
1 parent 07429d3 commit b76c024
Showing 1 changed file with 5 additions and 4 deletions.
9 changes: 5 additions & 4 deletions salmon_tutorial.rst
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Expand Up @@ -47,12 +47,13 @@ Salmon requires that paired reads be separated into two files. We can split the

Now, we can quantify our reads against this reference:
::
for file in *.pe.fq.gz.1
for file in *.pe.fq.gz.1.fq
do
tail=.abundtrim.subset.pe.fq.gz.1
BASE=${file/$tail/}
tail1=.abundtrim.subset.pe.fq.gz.1.fq
tail2=.abundtrim.subset.pe.fq.gz.1.fq
BASE=${file/$tail1/}
salmon quant -i transcript_index --libType IU \
-1 $BASE$tail -2 $BASE$tail -o $BASE.quant;
-1 $BASE$tail1 -2 $BASE$tail2 -o $BASE.quant;
done
(Note that --libType must come before the read files!)

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