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Update salmon_tutorial.rst
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halexand committed Oct 12, 2016
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6 changes: 3 additions & 3 deletions salmon_tutorial.rst
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Expand Up @@ -40,22 +40,22 @@ Create the salmon index:

Salmon requires that paired reads be separated into two files. We can split the reads using the XXX script XXX: *CHECK ME!*
::
for file in *.abundtrim.subset.pe.fq
for file in *.abundtrim.subset.pe.fq.gz
do
split-reads.py $file
done

Now, we can run our reads against this reference:
::
for file in *1.fq
for file in *1.fq.gz
do
BASE=${file/.1.fq/}
salmon quant -i transcript_index --libType IU \
-1 $BASE.1.fq -2 $BASE.2.fq -o $BASE.quant;

(Note that --libType must come before the read files!)

This will create a bunch of directories named after the fastq files that we just pushed through. Take a look at what files there are within one of these directories: **FIX**
This will create a bunch of directories named after the fastq files that we just pushed through. Take a look at what files there are within one of these directories:
::
find SRR1976948.quant -type f

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