Merge pull request #4 from marbre2/patch-1

added detailed instruction to the README.md

README.md

@@ -11,7 +11,11 @@ from ABBA-registered data stored in QuPath projects.
 
 ## Installation
 
-
+
+### Python version
+This software has been tested on **Python 3.9**
+
+
 ### First-Time Installation
 ```
 pip install ABBA-QuPath-RegistrationExporter
@@ -24,14 +28,63 @@ pip install ABBA-QuPath-RegistrationExporter
 pip install --upgrade ABBA-QuPath-RegistrationExporter
 ```
 
-## Running
-
+## Running Software
+To launch the programm and open the GUI
 ```
 regexport
 ```
 
+### How to use
+1. **Download BIOP Extensions**
+
+Provide empty directory to download required files from BIOP
+
+2. **Get QuPath Exporter**
+
+Download and Save the .groovy script to download and to save .tsv file from QuPath.
+
+Please provide a name and save it to the destination directory.
+
+3. **Load Brainglobe Atlas**
+
+When using for the first time, this button downloads the Brainglobe Atlas - it is currently using the **allen_mouse_25um** version.
+
+Press the button to display the 3D view of the atlas and to load the whole hierarchy of the brain regions.
+
+4. **Load TSV Files**
+
+Provide the path the .tsv file(s) to load in the software, multiselection is required for multiple sections viewing.
+
+5. **Use the left-hand panel**
+
+When clicking on the brain (sub)region name, it will only display cells located in the chosen region.
+
+Multiselection is possible to display different regions simultaneously.
+
+6. **Use the right-hand panel** 
+
+* First drop-down menu is to select according to which criteria the colormap will be applied.
+
+e.g. BrainRegion -> 1 color per different region
+
+e.g. CustomColumns (from the .tsv file) -> 1 color per different value
+
+* Second drop-down menu is to select the colormap.
+* Figure displays Histogram and cumulative histogram from the selected content in the first drop-down menu.
+* Sliders are to set up threshold according for each CustomColumns, and to ignore cells that are above those thresholds. The *ignored* cells will then be OFF on the brain atlas visualization. **Only the cells that are visualized on the atlas will be saved in the last step**
+
+7. **Save Cells**
+
+Provide the name and path to save the output result as .csv or .feather file. *.feather file is a compressed version of .csv*
+By default, it is saving the follogin content for each cells (position + custom columns + acronym/name of the finest sub-region area in the brain atlas + original image/.tsv file).
+
+You can choose to save **Selected cells only**, in that case it is saving only the result for the displayed cells. The Acronym and BrainRegion fields will be the one selected in the hierarchy in the left-hand panel.
+
 ## Running Tests
 
 ```
+git clone https://github.com/nickdelgrosso/ABBA-QuPath-RegistrationAnalysis
+cd ABBA-QuPath-RegistrationAnalysis
+pip install -e .[dev]
 python -m pytest
 ```