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uM2 gamma pixel size doesn't update with Ti2 tube lens #78

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samjlord opened this issue Oct 21, 2019 · 5 comments
Open

uM2 gamma pixel size doesn't update with Ti2 tube lens #78

samjlord opened this issue Oct 21, 2019 · 5 comments

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@samjlord
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When I change the tube lens on the Ti2, uM2 gamma doesn't update the pixel size, unless I press the "refresh" button. In uM1.4, the pixel size changes instantaneously. When using our old Ti scope with an Arduino- and magnet-based tube lens sensor, the pixel size updates immediately in uM2 gamma. For the Ti2, the tube lens property is not included in the pixel size calibration.

(sorry, I had posted this at the older repository before: https://github.com/micro-manager/micro-manager/issues/676)

@nanthony21
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The magnification on the TI2 seems to work ok for me.

Where are you checking what your current pixel size is? For me, I check the grid creator window in the position list, each time I open it it shows the correct size accounting for the current magnification and binning settings.

@samjlord
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samjlord commented Nov 9, 2019

If I snap an image, change the mag to 1.5x, then snap another image, the frame size in the top of the image window does not update. I need to first close that window and snap a new image to get the correct size in microns. Also, the pixel size listed on the main uM window does not update unless I press the "refresh" button.

@nanthony21
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I see. Have you checked the image metadata to see if the pixel size is being saved correctly? If the metadata is saving correctly and this is just a matter of the GUI labels not updating properly then I imagine this probably won't be a priority for most people.

@samjlord
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The metadata is not saved correctly unless you first press the reset button.

@nanthony21
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Hmm, that does seem like an issue that should be fixed. Unfortunately the solution probably lies inside the device adapter for the TI2 which is closed-source. I'm not really sure how the closed-source code is handled, is this something that Nikon needs to fix?

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