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use HMM-based classification of TEsorter only
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oushujun committed May 8, 2023
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8 changes: 4 additions & 4 deletions LTR_retriever
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Expand Up @@ -5,11 +5,11 @@ use File::Basename;

##Supported dependent program versions (or up): GenomeTools/1.5.1, BLAST+/2.2.25, BLAST/2.2.25, HMMER/3.1b1, RepeatMasker/3.3.0, CDHIT/4.5.6, Tandem Repeats Finder 4.07b, and Perl 5.0.0

my $version="v2.9.3";
my $version="v2.9.4";
my $help="
##########################
############################
### LTR_retriever $version ###
##########################
############################
A program for accurate identification of LTR-RTs from outputs of LTRharvest and
LTR_FINDER, generates non-redundant LTR-RT library for genome annotations.
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`${hmmer}hmmsearch --tblout $index.retriever.scn.extend.fa.aa.tbl --notextw --cpu $threads -E 0.05 --domE 0.05 --noali $TEhmm $index.retriever.scn.extend.fa.aa > $index.retriever.scn.extend.fa.aa.scn`;
`touch $index.retriever.scn.extend.fa.aa.tbl` unless -s "$index.retriever.scn.extend.fa.aa.tbl";
`perl $script_path/bin/annotate_TE.pl $index.retriever.scn.extend.fa.aa.tbl > $index.retriever.scn.extend.fa.aa.anno`;
`$TEsorter $index.retriever.scn.extend.fa -p $threads 2>/dev/null`;
`$TEsorter $index.retriever.scn.extend.fa --disable-pass2 -p $threads 2>/dev/null`;
`awk '{print \$1"\\t"\$2"\\t"\$3"\\t"\$6"\\t"\$7}' $index.retriever.scn.extend.fa.rexdb.cls.tsv >> $index.retriever.scn.extend.fa.aa.anno`;

# identify intact LTR retrotransposons
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