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isoseq for scRNA: UMI with homopolymers are not removed #692

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JiangyanYu opened this issue May 24, 2024 · 1 comment
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isoseq for scRNA: UMI with homopolymers are not removed #692

JiangyanYu opened this issue May 24, 2024 · 1 comment
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@JiangyanYu
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Operating system
Linux

Package name
smrtlinks v13

Conda environment
not used in installation

Describe the bug
UMI AAAAAAAAAAAA are found in the annotated.info.csv.

Error message
not a program error.

To Reproduce
grep AAAAAAAAAAAA in annotated.info.csv

Expected behavior
In the 10x cellranger pipeline, molecules with homopolymers UMI are removed, might be due to low quality. Can you please explain further why these molecules are included in the isoseq pipeline? Thanks very much!

@zeeev zeeev added the IsoSeq label May 29, 2024
@armintoepfer
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You need to remove them on their own.

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3 participants