From the input, REMP takes the nucleotide sequence of a DNA primer carrying a desired mutation and instantaneously generates its degenerate sequences having restriction sites that are 6-8 base pairs in length. User can easily edit the list of restriction sites (table 2) to be analyzed by REMP.
REMP can be executed by in one of the two following ways.
- Using executable file
- Using python code
REMP executable files for WindowsOS and MacOS can be download from here. The downloaded compressed folder contains three files, one is an application file REMP and two are data files, table 1 and table 2. Run the REMP.exe file to launch the application in WindowsOS. To launch the REMP in MacOS, type the below commands in the terminal.
$cd Downloaded Path/REMP Mac/
$./REMP
Clone or download this repo to a local machine. REMP can be run on any operating system having a Python 3 compiler that is required to run the program. To run the REMP software, enter the below commands in the command prompt/terminal.
$ cd Downloaded Path/REMP/
$ python3 REMP.py
REMP is under MIT license. For more information, click here.