WEAP: Whole Exome Analysis Pipeline

Operating system(s): Ubuntu 20.04 and 22.04.

Programming language used: Bash

Important: Check bash in your linux using the command:

       which bash

Desired output:

       /usr/bin/bash

Other requirements: Java 11, Python3, GLIBC 2.28 or higher.

Executable Binaries are converted from bash using Shell Script Compiler (SHC).

Developer: Ranjan Jyoti Sarma and Prof. N. Senthil Kumar

1.Downloading WEAP

i. Install git using:

  sudo apt -y install git

ii. Change the current directory to Desktop:

  cd  ~/Desktop

iii. Download WEAP using git:

   git clone https://github.com/ranjanjs34/weap.git

iv. After downloading, enter into the WEAP:

   cd WEAP

NOTE: Advanced users may keep WEAP in any convenient location.

2.Setting Up WEAP:

Add execution permission to binary files configure and reference:

   sudo chmod  +x ./configure
   sudo chmod  +x ./reference

3.Downloading prerequisite tools [One Time]

To download the prerequisite tools, run the configure as :

   ./configure

Enter the directory address where it downloads the pre-requisite tools: for example: /home/$USER/Desktop

There should not be any ‘/’ at the end of the directory address.

This Script will automatically download and install the required tools and will add to the path so that WEAP can recognize the internal commands. As ANNOVAR cannot be distributed, users need to download the ANNOVAR from the ANNOVAR website after registration. Please download the ANNOVAR from https://www.openbioinformatics.org/annovar/annovar_download_form.php manually by registering, unzip and keep it in Tool directory as 'annovar'.

4.Downloading Reference Data [One Time]:

   ./reference

This will download the reference genome, Variants with Allele frequency from genomAD project, dbSNP data from gatk bundle and datasets from ANNOVAR web resource to annotate the variants.

5.How to Run WEAP for variant calling (WEAP will guide the users at each step for the input):

There should not be any ‘/’ at the end of the input and output directory address.

  ./WEAP