SPC

We performed a computational analysis on the first 70 aa at the N-t (N-terminal analysis) of the whole proteome and on 70 aa windows starting from all internal TMDs (Internal TMDs analysis) to identify potential SPC cleavage sites not associated with signal peptides (cryptic cleavage sites) by using a pre-existing a signal peptide prediction software (SignalP) and comparing the output of two networks (no-TM and TM modes). Our hits represent membrane protein with type-II oriented TMD predicted to be cleaved in no-TM network mode but not in TM network mode. Moreover, we rank the hits based on the number of disease-linked mutation.