This is a major update of the Probe Maker.

Major changes from previous version:

A vastly-improved user interface with less clutter and code facing the user
We have switched to solely using Python 3.x, and will discontinue support for Python 2.7 from here on.
- This update does have dependencies that weren't required in the previous iterations, including Biopython, Numpy, and Pandas. BLAST+ is an optional dependency that enables full functionality of the program, but is not required for basic probe pair creation.
New functionality including the ability to pick between sets of probes, set limits on allowed homopolymer stretches, support for BLASTing probes to a reference .fasta file, and to drop possibly problematic probe pairs automatically.
An option to maximize coverage of a cDNA while keeping the number of pairs within the lowest IDT OPool price tier.
We have expanded the number of Initiator/Amplifier pairs to include the least cross-reactive published by T. Wang et al in their 2020 BioRxiv preprint.
- As a result it is theoretically possible to multiplex 13 probe sets (limited by fluorophore visualization ability).
We have added additional outputs to allow easier note keeping, supplemental figure making, and visualization of the probe locations relative to the cDNA of interest.