run_mlm function process

[elektrafying2000]

Hi! I was wondering if you could help me understand how the multivariate linear model works for pathway activity inference. I understand that the independent variables are interaction weights and the dependent variable the gene expression. But if someone could write a step-by-step of the function's process from input to output, that would be greatly appreciated!:)

[PauBadiaM]

Hi @elektrafying2000,

I previously explained how the Univariate Linear Model (ulm) works in this thread. The Multivariate Linear Model (mlm) is just an extension of ulm, the difference is that it uses all pathways at the same time during modeling, instead of doing it one by one like ulm. Hope this is helpful, please let me know if you have more questions.

[elektrafying2000]

Thank you so much for the insight and for taking the time to explain! The explanation was super clear:).