Use SCTransform

[pabloatria18]

Hello
I want to run scTransform on my data.
I have two different conditions, so I want to integrate them
If I merge both of my seurat objects previous to any analysis gives me an error.

I am trying to do the following:

my.data.control <- Read10X(data.dir = "C:/Users/Pablo/Documents/RNA-seq/R/count-Control/10")
my.data.loaded <- Read10X(data.dir = "C:/Users/Pablo/Documents/RNA-seq/R/count-Loaded/10")
my.data <- CreateSeuratObject(counts = my.data.control, project = "CL", min.cells = 3, min.features = 200)
my.data2 <- CreateSeuratObject(counts = my.data.loaded, project = "CL", min.cells = 3, min.features = 200)

pbmc <- my.data
pbmc2 <- my.data2
group <- sample(c("control"), size = 5797, replace = TRUE)
group2 <- sample(c("loaded"), size = 4705, replace = TRUE)

names(group) <- colnames(pbmc)

names(group2) <- colnames(pbmc2)

pbmc <- AddMetaData(object = pbmc, metadata = group, col.name = "group")
pbmc2 <- AddMetaData(object = pbmc2, metadata = group2, col.name = "group")
pbmc.combined <- merge(pbmc, pbmc2, add.cell.ids = c("Control", "Loaded"), project = "Test")

pancreas.list <- SplitObject(pbmc.combined, split.by = "group")
pancreas.list <- pancreas.list[c("Control", "Loaded")]
for (i in 1:length(pancreas.list)) {
pancreas.list[[i]] <- SCTransform(pancreas.list[[i]], verbose = FALSE)
}

But I am getting this message:
Error in h(simpleError(msg, call)) :
error in evaluating the argument 'x' in selecting a method for function 't': missing value where TRUE/FALSE needed
In addition: There were 27 warnings (use warnings() to see them)

Thanks for the help

[jaisonj708]

This will likely resolve after installing the development version of sctransform: remotes::install_github("ChristophH/sctransform@develop")

Also see the following related issue: satijalab/sctransform#71