This repository contains Primary Data (except files containing patient data) and the R code used in the analyses for our manuscript entitled:
Ultrasensitive chimerism enhances measurable residual disease testing after allogeneic hematopoietic cell transplantation
Sami B. Kanaan, Francesca Urselli, Jerald P. Radich, and J. Lee Nelson
Published in 2023 in Blood Advances, follow this link: https://doi.org/10.1182/bloodadvances.2023010332
Moreover, this repository contains Experimental User Guidelines, and Templates for planning, running, and analyzing Ultrasensitive Chimerism quantification experiments.
Information about the HLA-specific assays, plus GSTT1, AT3, and TG genomic regions and their designed molecular assay oligonucleotides (primers and fluorogenic probes) are available in patent US 10,604,805-B2. Those of TNN and the “Chi” series of assays are undisclosed and awaiting patent filing; but, these can be shared upon reasonable request. Please contact the study's corresponding author (owner of this repository) at: skanaan@chimerocyte.com
The goal is to make it transparent for anyone who wishes to use our experiemental and analytic setup, for them to apply on their own projects and data.