SIDESPLITTER plugin

This plugin provides a wrapper for SIDESPLITTER program for mitigating local overfitting.

Installation

You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:

1. Stable version

scipion installp -p scipion-em-sidesplitter

2. Developer's version

   • download repository

   git clone -b devel https://github.com/scipion-em/scipion-em-sidesplitter.git

   • install

   scipion installp -p /path/to/scipion-em-sidesplitter --devel

SIDESPLITTER sources will be installed automatically with the plugin, but you can also link an existing installation.

• Default installation path assumed is software/em/sidesplitter-1.2, if you want to change it, set SIDESPLITTER_HOME in scipion.conf file pointing to the folder where the SIDESPLITTER is installed.

To check the installation, simply run one of the following Scipion tests:

scipion test sidesplitter.tests.test_protocol_sidesplitter.TestSideSplitter

A complete list of tests can also be seen by executing scipion test --show --grep sidesplitter

Supported versions

1.2

Protocols

• local filter

References

1. Kailash Ramlaul, Colin M. Palmer and Christopher H. S. Aylett (2020). Mitigating Local Over-fitting During Single Particle Reconstruction with SIDESPLITTER. https://www.biorxiv.org/content/10.1101/2019.12.12.874081v2