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[Feature suggestion][amplicon] primer-pair searching limit #349

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Subject-13 opened this issue Nov 18, 2022 · 2 comments
Open
4 tasks done

[Feature suggestion][amplicon] primer-pair searching limit #349

Subject-13 opened this issue Nov 18, 2022 · 2 comments

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@Subject-13
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Subject-13 commented Nov 18, 2022

Hello!

Might have an idea for your tool:

Prerequisites

  • make sure you're are using the latest version by seqkit version
  • read the usage

Describe your issue

  • describe the problem

It might be useful to collect only a certain number of hits for a primer pair from the given table and then remove the primer pair from the list so that it is no longer searched for in the reads.

  • provide a reproducible example

If I already have 1.000 hits for a particular primer pair, it is no longer necessary to search for the pair. Search in the reads until the limit for each pair is reached or all reads have been searched.

@shenwei356
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Owner

It does reduce search time when using many primers, but most of the time, we need to search all reads.

@Subject-13
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We could use this for our workflow since the nanopore sequencer provides a lot of data, but a certain amount of it becomes redundant for us and just costs time.

I would try to do this adaptation by myself and could give you feedback if it's stable.

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