Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging
LaTeX source code for my paper in the Journal of Neuroscience Methods comparing awake and anesthetized cerebral blood flow measurements with multi-exposure speckle imaging (MESI). This work was conducted during my postdoc at the University of Texas at Austin in the Functional Optical Imaging Laboratory (@UTFOIL).
https://doi.org/10.1016/j.jneumeth.2021.109434
Colin T. Sullender, Lisa M. Richards, Fei He, Lan Luan, and Andrew K. Dunn, "Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging," Journal of Neuroscience Methods, 109434 (2022). doi: 10.1016/j.jneumeth.2021.109434
BibTeX
@article{SULLENDER2021109434,
title = {Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging},
journal = {Journal of Neuroscience Methods},
volume = {366},
pages = {109434},
year = {2022},
issn = {0165-0270},
doi = {https://doi.org/10.1016/j.jneumeth.2021.109434},
url = {https://www.sciencedirect.com/science/article/pii/S0165027021003691},
author = {Colin T. Sullender and Lisa M. Richards and Fei He and Lan Luan and Andrew K. Dunn},
keywords = {Multi-exposure speckle imaging, Laser speckle contrast imaging, Awake imaging, Anesthesia, Hemodynamics, Cerebral blood flow},
abstract = {Background
Anesthetized animal models are used extensively during neurophysiological and behavioral studies despite systemic effects from anesthesia that undermine both accurate interpretation and translation to awake human physiology. The majority of work examining the impact of anesthesia on cerebral blood flow (CBF) has been restricted to before and after measurements with limited spatial resolution.
New method
We used multi-exposure speckle imaging (MESI), an advanced form of laser speckle contrast imaging (LSCI), to characterize the dynamics of isoflurane anesthesia induction on cerebral vasculature and blood flow in the mouse brain.
Results
The large anatomical changes caused by isoflurane are depicted with wide-field imagery and video highlighting the induction of general anesthesia. Within minutes of exposure, both vessel diameter and blood flow increased drastically compared to the awake state and remained elevated for the duration of imaging. An examination of the dynamics of anesthesia induction reveals that blood flow increased faster in arteries than in veins or parenchyma regions.
Comparison with existing methods
MESI offers robust hemodynamic measurements across large fields-of-view and high temporal resolutions sufficient for continuous visualization of cerebrovascular events featuring major changes in blood flow.
Conclusion
The large alterations caused by isoflurane anesthesia to the cortical vasculature and CBF are readily characterized using MESI. These changes are unrepresentative of normal physiology and provide further evidence that neuroscience experiments would benefit from transitioning to un-anesthetized awake animal models.}
}
- 2021-12-01: Paper published online in Journal of Neuroscience Methods
- 2021-11-29: Manuscript accepted for publication in Journal of Neuroscience Methods
- 2021-11-08: Revisions submitted to Journal of Neuroscience Methods (Manuscript v3.0)
- 2021-08-31: Updated bioRxiv submission (bioRxiv v2.0): https://doi.org/10.1101/2020.06.26.174227
- 2021-08-30: Submitted to Journal of Neuroscience Methods (Manuscript v2.0)
- 2020-08-30: Rejected from Neurophotonics 😭
- 2020-06-30: Resubmitted to Neurophotonics after revising to structured abstract format (Manuscript v1.1)
- 2020-06-26: Uploaded to bioRxiv (bioRxiv v1.0): https://doi.org/10.1101/2020.06.26.174227
- 2020-06-26: Submitted to Neurophotonics (Manuscript v1.0)
© 2021 Colin T. Sullender, Lisa M. Richards, Fei He, Lan Luan, and Andrew K. Dunn