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Update README.md
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pas2182 committed Mar 7, 2020
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Expand Up @@ -22,5 +22,5 @@ Suggested usage:

python umap_transform.py -rm REFDATA/REFDATA.matrix.txt -pm QUERY1/QUERY1.matrix.txt QUERY2/QUERY2.matrix.txt -p project_to_REFDATA/project_to_REFDATA --markers markers.txt -k 5

where REFDATA.matrix.txt is a matrix of molecular counts for the reference (first two columns contain GIDS and gene symbols, subsequent column contain counts for each cell), QUERYX.matrix.txt is a matrix of molecular counts for query sample X (same format as REFDATA.matrix.txt), markers.txt is a one-column list of GIDS for computing similarity (usually highly variable genes).
where REFDATA.matrix.txt is a tab-delimited matrix of molecular counts for the reference (first two columns contain GIDS and gene symbols, subsequent column contain counts for each cell), QUERYX.matrix.txt is a matrix of molecular counts for query sample X (same format as REFDATA.matrix.txt), markers.txt is a one-column list of GIDS for computing similarity (usually highly variable genes). There should be no header in any of the files.

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