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give exact plate model used
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astraw committed Sep 22, 2023
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Expand Up @@ -63,11 +63,9 @@ LEE filter 738 is used for blocking the excitation light. Whole optic design of

**Figure 4.** Optic design for Miriam plus to detect Syto9 + DNA.

The middle heating board and the LED and PD arrangements have been designed to support 96-well plates with the following dimensions (figure 5).

![](96well.PNG)

**Figure 5.** 96-well plate dimensions supported by Miriam
The middle heating board and the LED and PD arrangements have been designed to
support the BioRad HSL9601 plates (96-Well PCR Plates, low profile, semi
skirted, clear/clear) with sealing film.

To control the heating of Miriam there are two heater PCBs. They have been designed so that in the middle is a 10 kohm SMD thermistor and the heat is generated by current flowing through the copper traces. These traces have been designed to be so long and narrow that they create an internal resistance of few ohms. The traces are closer to each other in the edges because heat is measured from the middle and the edges of the PCB boards meet ambient air and they need to produce more heat. (Figure 6.)

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