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Media
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Media
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MEDIA (“SDMM”, [CYS,ASN,GLN,ADENINE,NIACINE,BIOTIN,PANTOTHENATE,PYRIDOXAL_PHOSPHATE,THIAMINE,RIBOFLAVIN,K,T,NA+2,ACET,
HCO3,MG,CL,CA,CU,ZN,CHOLINE,FE+2,MN])
#ltrp not found / ca-pathothenate / pyridoxine HCL (pyridoxal phosphate) / sulfate?
#t is phosphate
MEDIA (“CDM”, [URACIL,ADENINE,PANTOTHENATE,NIACINE,THIAMINE,RIBOFLAVIN,BIOTIN,PHOSPHATE,K,NA+2,ACET,HCO3,MG,CL,CA,CU,ZN,
MN,L-ALPHA-ALANINE,ARG,ASN,ASP,CYS,GLN,GLU,GLY,HIS,ILE,LEU,LYS,MET,PHE,PRO,SER,THR,TRP,TYR,VAL,Glucose,CHOLINE,FE+2])
#D-ALANINE or L-ALPHA-ALANINE
MEDIA ("MMDCM", [URACIL,ADENINE,NIACINE,K,NA+2,MG,CL,CA,CU,ZN,MN,ARG,CYS,GLN,GLY,HIS,ILE,LEU,MET,PRO
SER,THR,VAL,Glucose,CHOLINE,FE+2])
#sulfate?? catalase??
#START GROWTH EXPERIMENTS IN CDM
Experiment(“D39_CDM-Ala”, control_media=CDM, experimental_media=CDM-Ala, control_strain=D39, experimental_strain=D39, growth_ratio=0.20433194)
Experiment(“D39_CDM-Arg”, control_media=CDM, experimental_media=CDM-Arg, control_strain=D39, experimental_strain=D39, growth_ratio=0.02453225)
Experiment(“D39_CDM-Asp”, control_media=CDM, experimental_media=CDM-Asp, control_strain=D39, experimental_strain=D39, growth_ratio=0.20403643)
Experiment(“D39_CDM-Cys”, control_media=CDM, experimental_media=CDM-Cys, control_strain=D39, experimental_strain=D39, growth_ratio=0.03645183)
Experiment(“D39_CDM-Gln”, control_media=CDM, experimental_media=CDM-Gln, control_strain=D39, experimental_strain=D39, growth_ratio=0.12028716)
Experiment(“D39_CDM-Glu”, control_media=CDM, experimental_media=CDM-Glu, control_strain=D39, experimental_strain=D39, growth_ratio=0.1989362)
Experiment(“D39_CDM-Gly”, control_media=CDM, experimental_media=CDM-Gly, control_strain=D39, experimental_strain=D39, growth_ratio=0.02897007)
Experiment(“D39_CDM-His”, control_media=CDM, experimental_media=CDM-His, control_strain=D39, experimental_strain=D39, growth_ratio=0.03583967)
Experiment(“D39_CDM-Ile”, control_media=CDM, experimental_media=CDM-Ile, control_strain=D39, experimental_strain=D39, growth_ratio=0.02323636)
Experiment(“D39_CDM-Leu”, control_media=CDM, experimental_media=CDM-Leu, control_strain=D39, experimental_strain=D39, growth_ratio=0.14422536)
Experiment(“D39_CDM-Lys”, control_media=CDM, experimental_media=CDM-Lys, control_strain=D39, experimental_strain=D39, growth_ratio=0.18948889)
Experiment(“D39_CDM-Met”, control_media=CDM, experimental_media=CDM-Met, control_strain=D39, experimental_strain=D39, growth_ratio=0.18929472)
Experiment(“D39_CDM-Phe”, control_media=CDM, experimental_media=CDM-Phe, control_strain=D39, experimental_strain=D39, growth_ratio=0.17606816)
Experiment(“D39_CDM-Pro”, control_media=CDM, experimental_media=CDM-Pro, control_strain=D39, experimental_strain=D39, growth_ratio=0.2067433)
Experiment(“D39_CDM-Ser”, control_media=CDM, experimental_media=CDM-Ser, control_strain=D39, experimental_strain=D39, growth_ratio=0.183829)
Experiment(“D39_CDM-Thr”, control_media=CDM, experimental_media=CDM-Thr, control_strain=D39, experimental_strain=D39, growth_ratio=0.19752675)
Experiment(“D39_CDM-Trp”, control_media=CDM, experimental_media=CDM-Trp, control_strain=D39, experimental_strain=D39, growth_ratio=0.15941508)
Experiment(“D39_CDM-Tyr”, control_media=CDM, experimental_media=CDM-Tyr, control_strain=D39, experimental_strain=D39, growth_ratio=0.2145561)
Experiment(“D39_CDM-Val”, control_media=CDM, experimental_media=CDM-Val, control_strain=D39, experimental_strain=D39, growth_ratio=0.01553346)
#give all a unique nam
# strain 19F
Experiment(“19F_CDM-Ala”, control_media=CDM, experimental media=CDM-Ala, control_strain=19F, experimental_strain=19F, growth_ratio=0.146333)
Experiment(“19F_CDM-Arg”, control_media=CDM, experimental media=CDM-Arg, control_strain=19F, experimental_strain=19F-Arg, growth_ratio=0.15182487)
Experiment(“19F_CDM-Asn”, control_media=CDM, experimental media=CDM-Asn, control_strain=19F, experimental_strain=19F, growth_ratio=0.03607297)
Experiment(“19F_CDM-Asp”, control_media=CDM, experimental media=CDM-Asp, control_strain=19F, experimental_strain=19F, growth_ratio=0.1577768)
Experiment(“19F_CDM-Cys”, control_media=CDM, experimental media=CDM-Cys, control_strain=19F, experimental_strain=19F, growth_ratio=0.02427277)
Experiment(“19F_CDM-Gln”, control_media=CDM, experimental media=CDM-Gln, control_strain=19F, experimental_strain=19F, growth_ratio=0.12752002)
Experiment(“19F_CDM-Glu”, control_media=CDM, experimental media=CDM-Glu, control_strain=19F, experimental_strain=19F, growth_ratio=0.14547937)
Experiment(“19F_CDM-Gly”, control_media=CDM, experimental media=CDM-Gly, control_strain=19F, experimental_strain=19F, growth_ratio=0.15276418)
Experiment(“19F_CDM-His”, control_media=CDM, experimental media=CDM-His, control_strain=19F, experimental_strain=19F, growth_ratio=0.05588467)
Experiment(“19F_CDM-Ile”, control_media=CDM, experimental media=CDM-Ile, control_strain=19F, experimental_strain=19F, growth_ratio=0.12415293)
Experiment(“19F_CDM-Leu”, control_media=CDM, experimental media=CDM-Leu, control_strain=19F, experimental_strain=19F, growth_ratio=0.02297063)
Experiment(“19F_CDM-Lys”, control_media=CDM, experimental media=CDM-Lys, control_strain=19F, experimental_strain=19F, growth_ratio=0.15612187)
Experiment(“19F_CDM-Met”, control_media=CDM, experimental media=CDM-Met, control_strain=19F, experimental_strain=19F, growth_ratio=0.07663296)
Experiment(“19F_CDM-Phe”, control_media=CDM, experimental media=CDM-Phe, control_strain=19F, experimental_strain=19F, growth_ratio=0.1452984)
Experiment(“19F_CDM-Pro”, control_media=CDM, experimental media=CDM-Pro, control_strain=19F, experimental_strain=19F, growth_ratio=0.14777776)
Experiment(“19F_CDM-Ser”, control_media=CDM, experimental media=CDM-Ser, control_strain=19F, experimental_strain=19F, growth_ratio=0.1463815)
Experiment(“19F_CDM-Thr”, control_media=CDM, experimental media=CDM-Thr, control_strain=19F, experimental_strain=19F, growth_ratio=0.14387735)
Experiment(“19F_CDM-Trp”, control_media=CDM, experimental media=CDM-Thr, control_strain=19F, experimental_strain=19F, growth_ratio=0.13990791)
Experiment(“19F_CDM-Tyr”, control_media=CDM, experimental media=CDM-Tyr, control_strain=19F, experimental_strain=19F, growth_ratio=0.13968132)
Experiment(“19F_CDM-Val”, control_media=CDM, experimental media=CDM-Val, control_strain=19F, experimental_strain=19F, growth_ratio=0.13069792)
# strain TIGR4
Experiment(“TIGR4_CDM-Ala”, control_media=CDM, experimental_media=CDM-Ala, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.17120025)
Experiment(“TIGR4_CDM-Arg”, control_media=CDM, experimental_media=CDM-Arg, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.04471474)
Experiment(“TIGR4_CDM-Asn”, control_media=CDM, experimental_media=CDM-Asn, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.18276277)
Experiment(“TIGR4_CDM-Asp”, control_media=CDM, experimental_media=CDM-Asp, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.18395004)
Experiment(“TIGR4_CDM-Cys”, control_media=CDM, experimental_media=CDM-Cys, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.01961422)
Experiment(“TIGR4_CDM-Gln”, control_media=CDM, experimental_media=CDM-Gln, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.04353862)
Experiment(“TIGR4_CDM-Glu”, control_media=CDM, experimental_media=CDM-Glu, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.16395977)
Experiment(“TIGR4_CDM-Gly”, control_media=CDM, experimental_media=CDM-Gly, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.09956597)
Experiment(“TIGR4_CDM-His”, control_media=CDM, experimental_media=CDM-His, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.07433383)
Experiment(“TIGR4_CDM-Ile”, control_media=CDM, experimental_media=CDM-Ile, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.16268069)
Experiment(“TIGR4_CDM-Leu”, control_media=CDM, experimental_media=CDM-Leu, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.01832489)
Experiment(“TIGR4_CDM-Lys”, control_media=CDM, experimental_media=CDM-Lys, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.18062739)
Experiment(“TIGR4_CDM-Met”, control_media=CDM, experimental_media=CDM-Met, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.11248514)
Experiment(“TIGR4_CDM-Phe”, control_media=CDM, experimental_media=CDM-Phe, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.17506658)
Experiment(“TIGR4_CDM-Pro”, control_media=CDM, experimental_media=CDM-Pro, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.16557553)
Experiment(“TIGR4_CDM-Ser”, control_media=CDM, experimental_media=CDM-Ser, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.16719834)
Experiment(“TIGR4_CDM-Thr”, control_media=CDM, experimental_media=CDM-Thr, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.18624213)
Experiment(“TIGR4_CDM-Trp”, control_media=CDM, experimental_media=CDM-Trp, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.18171011)
Experiment(“TIGR4_CDM-Tyr”, control_media=CDM, experimental_media=CDM-Tyr, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.19145178)
Experiment(“TIGR4_CDM-Val”, control_media=CDM, experimental_media=CDM-Val, control_strain=TIGR4, experimental_strain=TIGR4, growth_ratio=0.13795122)
#name of each reaction should be unique!!