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TSS enrichment with a region extension 2000bp #1638

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Montsecb opened this issue Feb 26, 2024 · 0 comments
Open

TSS enrichment with a region extension 2000bp #1638

Montsecb opened this issue Feb 26, 2024 · 0 comments

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@Montsecb
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Hi there,
I was trying to calculate the TSS enrichment score using signac version 1.11 by adding an extension up to 2000 bp from TSSs. I see that the results produced have a high enrichment value (up to 150 in some cells). I would like to know if these values are normal or if they are too high, as I usually see enrichment values ranging up to 60 or 80.

I'm leaving the code and the results here using 1000 and 2000 bp.

seu <- TSSEnrichment(object = seu, fast=F, region_extension = 2000)

Here are the results using a region extension of 1000 bp(left) and 2000 bp (right)
Captura de Pantalla 2024-02-26 a las 9 41 56

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