Cobra

Cobra is an optimizer for Python bytecode. It performs several mini-optimizations on ASTs, many of which are from Python byte-code and micro-optimization. It can be downloaded using pip (pip install cobra-opt) and used like so:
Optimize a single Python file: python3 -m cobra-opt file.py
Optimize a folder of Python files: python3 -m cobra-opt -d directory/ or python3 -m cobra-opt --directory directory/