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timotheelionnet/DNAFISH_analysis
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A series of scripts for DNA FISH analysis, co-developed by Yi Fu and Timothee Lionnet The master script is DNA_FISH_pipeline.m, which exacute the following: generate_test_img.m Generate test images using functions in qc. DNA_FISH_pipeline_1.m Input raw DNA FISH images, perform Airlocalize (including clean up double detections) and crop loci from images. match_DNA_FISH_spots_between_channels.m Input cell mask images and .loc3 of centers of cropped loci, match up loci between different FISH channels according to cell ID and distances. analysis_overlap_between_loci.m Input loci match results and cropped loci images, compute cross correlation between match loci pairs. mensure_cropped_loci.m Input cropped loci images, compute centroid locations, radio of gyrations, and volumns of loci. plotPDF02032021.m Input data, plot PDF and CDF. analysis_cell_cycle.m Input loci match results, identify cell cycle(G1/G2) by number of DNA FISH spots, then plot DAPI/EU/IF intensity and distances between loci center grouped by cell cycle. *fileset, useful, and AIIRLOCALIZE1_stable is too big for uploading
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