lcos_multispot_pattern

Create a multispot pattern via phase modulation using a LCOS-SLM.

The repo contains:

• patternlib.py: the library for generating the multispot pattern.
• pattern_server.py: a script to start a "pattern server" listening for pattern parameters and returning 2D arrays containing the pattern. This server is used by LCOS_LabVIEW to obain LCOS patterns (LabVIEW and python communicate via a local socket).
• installer.cfg: configuration for building an NSIS installer for a stand-alone installation of pattern_server.py using pynsist.

The other files are just notebooks GUIs for playing around with generating the pattern and visualizing it using QT5 or jupyter notebook widgets.

Dependencies

• python 3.5+
• numpy 1.10+
• pyyaml 3.12

Cite

If you use this code, please cite the paper:

48-spot single-molecule FRET setup with periodic acceptor excitation
A. Ingargiola, M. Segal, A. Gulinatti, I. Rech, I. Labanca, P. Maccagnani, M. Ghioni, S. Weiss, X. Michalet
bioRxiv 156182; 2017. doi: https://doi.org/10.1101/156182

---

Copyright (C) 2017 The Regents of the University of California, Antonino Ingargiola and contributors.

This work was supported by NIH grants R01 GM069709 and R01 GM095904.