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File .err: why all my features have inconsistent gene locations #234

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esthercamilo opened this issue May 17, 2017 · 1 comment
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@esthercamilo
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esthercamilo commented May 17, 2017

I am not sure if here is the best place to put this question because it is conceptual (and not an issue about the program). I am using PROKKA with the following set of arguments:

$ prokka myorganism.fa --cdsrnaolap ON --rfam 1 --rawproduct

While my gbk and gff seems okay, I obtain an error file of 1.2Mb saying that all my features have inconsistent gene locations and "DiscRep_SUB:FEATURE_LOCATION_CONFLICT::Coding region xref gene does not exist".

I thought that may be because my genome is new the IDs are not recognized. Is that right?

@tseemann tseemann self-assigned this Aug 6, 2017
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tseemann commented Aug 6, 2017

I don't really understand tbl2asn errors anymore. NCBI keep "changing the rules". I will be removing it from the next release of Prokka.

PS. The syntax --cdsrnaolap ON is incorrect, just use --cdsrnaolap

@tseemann tseemann closed this as completed Aug 6, 2017
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