molbit sequences

[enovoa]

Hello! Thanks for sharing your work in bioRxiv. I was wondering if you could make available the fasta sequences for all 96 molbit sequences after 31 iterations of the evolutionary model? I could find the fasta with the corresponding squiggles in Supp Figure 5, but a plain txt/csv file with the individual fasta sequences would be very useful. Where can I find this? Thanks so much, and congrats on the preprint! Eva

[kdoroschak]

Hi Eva,

Thanks for reaching out! You can find the sequences here: https://github.com/uwmisl/Porcupine/blob/master/porcupine_sequences.fa Is this what you had in mind? We intend to include this as a supplementary file with the final publication as well, so it's easier to find.

Just want to note -- If you end up using any of these sequences for your RNA barcoding, I would recommend selecting those with read counts that hovered around the median (e.g. 11, 29, 37, 44, 67 as in Supp. Fig 8 in the preprint), due to the still-unexplained variation in read counts. That way no correction should be needed. (Only making this suggestion since we'd talked briefly about sequences at LC19. If you use these in any way, I would be curious to know how it goes!)

Best,
Katie

[enovoa]

Hi Katie,
Thank you very much! Yes, that's exactly what I was looking for :)
Yes, I agree would be useful to place them as supp File in the final publication, as I was unable to find them when looking for them.
Will definetely keep you guys posted on how it goes if we use them for RNA :) We will probably do a small trial first.
Thanks again!
Eva