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Kaiju script #13

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valeriafloral opened this issue Jan 5, 2021 · 0 comments
Closed

Kaiju script #13

valeriafloral opened this issue Jan 5, 2021 · 0 comments

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@valeriafloral
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Hi,
I assume that your file with right mates and the file with left mates contains the same number of reads and the reads are in the same order in both files.

Then you can just run Kaiju twice and combine the output of both runs.

First, run Kaiju with the intact read pairs in paired-end mode:

kaiju -i leftmates.fastq -j rightmates.fastq -o pairedreads.out ...

Second, run the singletons:

kaiju -i singletons.fastq -o singletons.out ...

Then just concatenate them

cat pairedreads.out singletons.out > combined.out

Now you can run kaijuReport or kaiju2krona etc on the combined.out file.

Originally posted by @pmenzel in bioinformatics-centre/kaiju#9 (comment)

@valeriafloral valeriafloral changed the title Kaiju Pared and unpaired Kaiju paired and unpaired Jan 5, 2021
@valeriafloral valeriafloral added this to To do in Metatranscriptomics via automation Jan 5, 2021
@valeriafloral valeriafloral changed the title Kaiju paired and unpaired Kaiju script Jan 5, 2021
Metatranscriptomics automation moved this from To do to Done Jan 27, 2021
@valeriafloral valeriafloral added help wanted Extra attention is needed and removed help wanted Extra attention is needed labels Jan 27, 2021
@valeriafloral valeriafloral removed this from Done in Metatranscriptomics Jan 28, 2021
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