Antibody-secreting cells are components of humoral immunity by secreting antibodies and providing protection against pathogens. These cells can be of IgM, IgA, or IgG subclass and migrate to class-specific niches. These cells' localisation and rareness make it challenging to define subclass-specific molecular hallmarks. Here, we describe how in-vitro differentiation of peripheral B-cells results in antibody-secreting cells. We use a single-cell multi-modal sequencing approach to find subclass-specific hallmark transcriptional profiles, surface protein expression and signaling pathway activity.
The pages contain code to process, analyze and create figures presented in the full manuscript:
- QC processes count tables (available at GSE189953): shows how to select high-quality cells; normalize and scale counts.
- PC haracterization describes the multi-modal characterization of 11-day-old differentiated B-cells to antibody-secreting cells.
- Hallmarks Ig-classes describes the identification of Ig-classes and corresponding mRNA, surface protein levels and signaling activity: (phospho-)protein levels.
We are very thankful for the efforts made by developers of Seurat, MOFA+ and workflowr. These (well-documented) R-packages enable respectively extensive multi-modal data analysis and reproducible code documentation.
The content in this repository is available under the CC BY 4.0 license.
For proper attribution, please cite our publication containing description and analysis of all presented data and results.