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Illumina now produces FASTQ without the /1 and /2, and instead uses a fasta description (ID, space, DESC).
@M00855:4:000000000-A16FH:1:1101:14529:1450 2:N:0:1 TGGGCAGCAGCGACTTCTGCCACAGTGTCGGTGACATGCCAAACGGTGGGT
When passing through the clip: tool, the DESC is discarde
d@M00855:4:000000000-A16FH:1:1101:14529:1450 GGGCAGCCTCAGCGCCCCGATGGGCGGAATGGGCCTGTCGGGCGT
ie. the "2:N:0:1" is missing.
The text was updated successfully, but these errors were encountered:
Description will now be retained by "clip:".
However, many tools in nesoni and outside of nesoni expect reads to be uniquely named (unless they are explicitly treated as pairs).
nesoni.io.check_name_uniqueness can be used to check input. This is currently used by "shrimp:" and now also by "clip:".
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Illumina now produces FASTQ without the /1 and /2, and instead uses a fasta description (ID, space, DESC).
@M00855:4:000000000-A16FH:1:1101:14529:1450 2:N:0:1
TGGGCAGCAGCGACTTCTGCCACAGTGTCGGTGACATGCCAAACGGTGGGT
When passing through the clip: tool, the DESC is discarde
d@M00855:4:000000000-A16FH:1:1101:14529:1450
GGGCAGCCTCAGCGCCCCGATGGGCGGAATGGGCCTGTCGGGCGT
ie. the "2:N:0:1" is missing.
The text was updated successfully, but these errors were encountered: